日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人正常細胞>CRL-2190 HK-2 人腎近曲小管上皮細胞.

CRL-2190 HK-2 人腎近曲小管上皮細胞.

簡要描述:CRL-2190 HK-2 人腎近曲小管上皮細胞.,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,
提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-15
  • 訪  問  量:1868

產品分類

Product Category

詳細介紹

CRL-2190 HK-2 人腎近曲小管上皮細胞.

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2Depositors: RA ZagerBiosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: kidney, cortex Tissue: proximal tubule Cell Type: human papillomavirus 16 (HPV-16) transformedCellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectinPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2190 HK-2 人腎近曲小管上皮細胞.Receptors:epidermal growth factor (EGF), expressedDNA Profile (STR):Amelogenin: X,Y CSF1PO: 13 D13S317: 9 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 9 TPOX: 8,9 vWA: 17,18Age:*****Gender:maleComments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). Virus produced by the PA317 cells was used to transduce primary PTCs. Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. The cell line appears to be derived from a single cell based on Southern and FISH analysis. The E6/E7 genes are present in the HK-2 genome as determined by PCR. The cells retain a phenotype indicative of well differentiated PTCs. They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. HK-2 cells are anchorage dependent. The cells will not grow in methylcellulose, soft agar or suspension. HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:0.05 mg/ml BPE - provided with the K-SFM kit5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.Subculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.


CRL-2190 HK-2 人腎近曲小管上皮細胞.
















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 日韩精品欧美一区二区三区 | 91色+91sesex| 青青操视频在线免费观看 | 久久99国产精品成人欧美 | 亚洲男人的天堂网站 | 成 人 a v免费视频 | 欧美xxxxbb| 91在线视频免费观看 | 久久青青草原精品影院 | 欧美不卡激情三级在线观看 | 和日本免费不卡在线v | 精品热99| 在线看日韩 | 日韩久久精品一区二区三区 | 无人视频免费观看免费视频 | 国产一卡2卡3卡4卡公司在线 | 成人精品一区二区久久 | 国产午夜一级淫片 | 国产自产精品 | 成人亚洲天堂 | 97欧美在线看欧美视频免费 | 国产精品99久久久久久董美香 | 国产成人在线视频网站 | 欧美久久超级碰碰碰二区三区 | 久久激情综合网 | 综合国产在线 | 久久青视频 | 四虎最新影院 | 午夜视频一区二区 | 国产香蕉精品视频在 | 日本高清免费在线视频 | 国产高清a毛片在线看 | 国产欧美视频一区二区三区 | 成人免费视频一区二区 | 91国偷自产一区二区三区蜜臀 | 亚洲精品乱码久久久久久蜜桃欧美 | 97免费观看视频 | 欧美第一页在线观看 | 欧美国产成人免费观看永久视频 | 国产欧美日韩第一页 | 亚洲黄网址 |