日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人正常細胞>CRL-2190 HK-2 人腎近曲小管上皮細胞.

CRL-2190 HK-2 人腎近曲小管上皮細胞.

簡要描述:CRL-2190 HK-2 人腎近曲小管上皮細胞.,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,
提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-15
  • 訪  問  量:1868

產品分類

Product Category

詳細介紹

CRL-2190 HK-2 人腎近曲小管上皮細胞.

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2Depositors: RA ZagerBiosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: kidney, cortex Tissue: proximal tubule Cell Type: human papillomavirus 16 (HPV-16) transformedCellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectinPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2190 HK-2 人腎近曲小管上皮細胞.Receptors:epidermal growth factor (EGF), expressedDNA Profile (STR):Amelogenin: X,Y CSF1PO: 13 D13S317: 9 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 9 TPOX: 8,9 vWA: 17,18Age:*****Gender:maleComments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). Virus produced by the PA317 cells was used to transduce primary PTCs. Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. The cell line appears to be derived from a single cell based on Southern and FISH analysis. The E6/E7 genes are present in the HK-2 genome as determined by PCR. The cells retain a phenotype indicative of well differentiated PTCs. They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. HK-2 cells are anchorage dependent. The cells will not grow in methylcellulose, soft agar or suspension. HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:0.05 mg/ml BPE - provided with the K-SFM kit5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.Subculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.


CRL-2190 HK-2 人腎近曲小管上皮細胞.
















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 午夜羞羞视频在线观看 | 国产一区二区三区在线影院 | 国产成人精品2021欧美日韩 | 99视屏 | 国产成人亚洲综合在线 | 国内91视频| 亚洲国产精品线观看不卡 | 国产精品视频久久久 | 国产视频h | 欧美成人天天综合天天在线 | 久久国产精品视频 | 毛片大片免费看 | 国产精品日本一区二区在线播放 | 午夜日本一区二区三区 | 直接进入免费看黄的网站 | 国产小视频精品 | 97成人精品视频在线播放 | 国产精品一区二区三区免费 | 国产精品亚洲综合网站 | 99毛片| 日产精品久久久一区二区 | 国产小视频在线观看免费 | 99热这里只有精 | 亚洲欧美日本国产综合在线 | 青青青在线视频免费 | 日韩国产三级 | 九九九九九九精品免费 | 青青草视频国产 | 午夜精品影院 | 九九热精品在线 | 理论亚洲区美一区二区三区 | 欧美国产伦久久久久 | 羞羞视频官网 | 牛牛精品国内免费一区 | 久久99精品久久久久子伦 | 日本h肉动漫在线观看免费 日本a中文字幕 | 欧美日韩色视频在线观看 | 日本国产一区在线观看 | 成人欧美一区二区三区黑人妖 | 欧美一区二区视频三区 | 免费在线观看污视频网站 |