日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人正常細胞>CRL-1730HUV-EC-C 人臍靜脈內皮細胞

HUV-EC-C 人臍靜脈內皮細胞

簡要描述:CRL-1730 HUV-EC-C 人臍靜脈內皮細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

  • 產品型號:CRL-1730
  • 廠商性質:生產廠家
  • 更新時間:2024-11-15
  • 訪  問  量:2236

產品分類

Product Category

詳細介紹

CRL-1730 HUV-EC-C 人臍靜脈內皮細胞


ATCC® Number:CRL-1730™    Price:$280.00
Designations:HUV-EC-C

Biosafety Level:1

Shipped:frozen

Medium & Seru
Organism:Homo sapi
m:
See Propagation

Growth Properties:adherent


ens (human)

Morphology:endothelial
HUV-EC-C 人臍靜脈內皮細胞


Source:Organ: umbilical vein

Tissue: vascular endothelium

Disease: normal

Cell Type: endothelial



Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1730

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37° 1:2 to 1:3 is recommended

Medium Renewal: Two to three times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase



Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443


CRL-1730















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 视频一区国产 | 成人午夜看片在线观看 | 玖玖色视频 | 四虎影视1304t| 国产一级做a爰片在线看免费 | 国产精品一区二区四区 | 国产在线不卡 | 国产欧美日韩中文字幕 | 日本人的色道免费网站 | 欧美人与动交片免费播放 | 国产自在自线2021 | 武侠古典第7页色综合 | 一区二区三区日韩精品 | 亚洲社区在线观看 | 日韩视频一区二区 | 九九这里有精品 | 激情亚洲天堂 | 成年网站未满十八禁毛片免费 | 欧美xart系列高清在线视频 | 天堂 在线最新版在线 | 欧美一区二区三区影院 | 欧美69精品国产成人 | 色综合久久久久久久久久久 | 狠狠干97| 亚洲日韩精品欧美一区二区一 | 久久国产精品免费 | 男人天堂2023 | 四虎永久网址影院 | 四虎免费在线播放 | 毛片视频在线免费观看 | 日本v视频 | 在线观看视频日韩 | 日韩精品亚洲专区在线观看 | 国产在线观看网站 | 国产啪精品视频网给免丝袜 | 青青偷拍视频 | 欧美激情一区二区三区在线 | 欧美日韩一级黄色片 | 青青青视频在线播放视频 | 久久99精品久久只有精品 | 亚洲国产日韩在线一区 |