日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2234SNU-449細(xì)胞, 人肝癌細(xì)胞

SNU-449細(xì)胞, 人肝癌細(xì)胞

簡(jiǎn)要描述:SNU-449細(xì)胞, 人肝癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供 *培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2234
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2026

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

SNU-449細(xì)胞, 人肝癌細(xì)胞(ATCC® CRL-2234)

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449細(xì)胞, 人肝癌細(xì)胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 午夜羞羞影院体验区60秒 | 青青草国产精品 | 和阿同居的日子在线3 | 91日韩| 欧美三级欧美一级 | 欧美日韩综合在线视频免费看 | 国产91小视频 | 成在线人免费视频一区二区三区 | 日韩欧美在线一级一中文字暮 | 国产成人三级视频在线观看播放 | 天堂网国产 | 青青草国产青春综合久久 | 免费大香伊蕉在人线国产 | 日韩免费高清一级毛片久久 | 爆操妹子 | 欧美亚洲综合另类 | 九色在线视频观看 | 国产精品999视频 | 性感美女被爆操 | 国产激情视频在线播放 | eeuss在线影院 | 欧美日一区二区三区 | 黄色成人在线播放 | 黄 色 毛片免费 | 三级视频大全 | 欧美日韩国产在线一区 | aaa毛片手机在线现看 | 成在线人永久免费视频下载 | 亚洲欧美国产另类视频 | 国产在线欧美日韩一区二区 | 一区二区成人国产精品 | 日韩欧美一二三 | 午夜视频网址 | 精品一区中文字幕 | 亚洲视频在线观看免费视频 | 亚洲人成综合 | 香蕉视频成人在线观看 | 黄片毛片在线看 | 91亚洲精品第一综合不卡播放 | 四虎在线最新地址公告 | 高清不卡一区二区 |