日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-111AN3 CA細(xì)胞, 人子宮內(nèi)膜腺癌細(xì)胞

AN3 CA細(xì)胞, 人子宮內(nèi)膜腺癌細(xì)胞

簡(jiǎn)要描述:AN3 CA細(xì)胞, 人子宮內(nèi)膜腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-111
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:1909

產(chǎn)品分類

Product Category

詳細(xì)介紹

AN3 CA細(xì)胞, 人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number:HTB-111™    Price:$399.00
Designations:AN3 CA

Depositors:CJ Dawe

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
AN3 CA細(xì)胞, 人子宮內(nèi)膜腺癌細(xì)胞

Tumorigenic:Yes

DNA Profile (STR):Amelogenin: X

CSF1PO: 12,14,15

D13S317: 12,14

D16S539: 10,14

D5S818: 11,14

D7S820: 7,10,7.1

THO1: 10,9.3

TPOX: 8,10

vWA: 14,20



Isoenzymes:AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2



Age:55 years

Gender:female

Ethnicity:Caucasian

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week


Preservation:Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 国产精品视屏 | 日本污视频网站 | 四虎影视国产精品一区二区 | 我的女儿韩国电视剧在线观看 | 成年人视频免费在线观看 | 97色资源| 青青青国产在线手机免费观看 | 成人a区| 日韩欧美在线播放 | 久99视频| 一区二区高清在线观看 | 九九热国产视频 | 国产91在线免费观看 | 在线不卡视频 | 最新在线防屏蔽国产一区 | 国产精品久久国产三级国不卡顿 | 欧美精品国产第一区二区 | 国产一级毛片在线 | 亚洲日本欧美日韩高观看 | 亚洲国产成人久久综合一区77 | 欧美日韩国内 | 国产色网 | 亚洲三级免费 | 97精品国产高清在线看入口 | 一区二区手机视频 | 甜甜的肉禽系统小说娱乐圈 | 91视频这里只有精品 | 日韩高清不卡 | 亚洲人成在线影院 | 亚洲毛片在线免费观看 | 青青青在线观看视频免费播放 | 日韩一区国产二区欧美三 | 久久3| 一级一级特黄女人精品毛片视频 | 亚洲第一网站 | 天堂a免费视频在线观看 | 色综合久久久久久888 | 在线免费观看亚洲 | 狠狠色伊人亚洲综合第8页 狠狠色网站 | 日本高清中文字幕 | 美日韩视频 |