日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-1933769-P細胞,人腎癌細胞系

769-P細胞,人腎癌細胞系

簡要描述:769-P細胞,人腎癌細胞系
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1933
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1796

產(chǎn)品分類

Product Category

詳細介紹

769-P細胞,人腎癌細胞系

ATCC® Number:CRL-1933™    Price:$338.00
Designations:769-P

Depositors:RD Williams

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney
Disease: renal cell adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
769-P細胞,人腎癌細胞系

Isolation:Isolation date: 1975

Tumorigenic:Yes

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 10,14

D16S539: 9,13

D5S818: 12

D7S820: 10,11

THO1: 6,9.3

TPOX: 8,11

vWA: 18



Cytogenetic Analysis:This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.

Age:63 years

Gender:female

Ethnicity:Caucasian

Comments:This line was derived from a primary clear cell adenocarcinoma.

The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.

They can be grown in soft agar.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:              
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:12 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:35 hrs

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020



References:22648: Williams RD, et al. In vitro c*tion of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed:1010528
22653: Williams RD, et al. In vitro c*tion of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 羞羞视频破解版免费观看 | 国产精品久久国产三级国不卡顿 | 精品动漫在线观看 | 亚洲国产欧美久久香综合 | aⅴ在线免费观看 | 4虎影院午夜在线观看 | 91视频 - 88av | 亚洲男人精品 | 日本欧美韩国一区二区三区 | 免费精品国产自产拍在 | 国产国产成人精品久久 | 我不卡在线影院 | 天天亚洲综合 | 国产成人精品2021欧美日韩 | 亚洲美女性生活视频 | 国产真实伦对白在线播放 | 在线免费看黄色片 | 敢死队4完整版在线观看 | 亚洲一区二区三区四区在线 | 中文japanese在线播放 | 久久综合五月天婷婷伊人 | 欧美区亚洲区 | 久久a热 | 四虎国产精品视频免费看 | 亚洲国产欧美视频 | 国产欧美日韩精品第二区 | 亚洲色图综合在线 | 国产不卡精品一区二区三区 | 玖玖草在线观看 | 三区在线视频 | 99re这里只有| 全黄a免费一级毛片人人爱 全国最大色成免费网站 | 成人高清在线观看 | 99在线观看视频 | h亚洲 | 欧美一区二区三区男人的天堂 | 色偷偷7777www人 | 日本www色高清视频 日本www色 | 欧美一级黄色片在线观看 | 四虎成人4hutv影院 | 亚洲社区在线 |