日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-5971 SNU-1 人胃癌細胞

CRL-5971 SNU-1 人胃癌細胞

簡要描述:CRL-5971 SNU-1 人胃癌細胞,
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞;
細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2283

產品分類

Product Category

詳細介紹

CRL-5971 SNU-1 人胃癌細胞

ATCC® Number: CRL-5971™ Price: $417.00

Designations: SNU-1

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-5971 SNU-1 人胃癌細胞

Isolation: Isolation date: 1984

Receptors: vasoactive intestinal peptide (VIP), expressed

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type O; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12,13

D13S317: 11,14

D16S539: 9,12

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,10

vWA: 14

Cytogenetic Analysis: SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t(1;4) (q24;q33), +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.

Age: 44 years adult

Gender: male

Ethnicity: Asian

Comments: SNU-1 was derived in 1984 by J. Park and associates from a poorly differentiated primary carcinoma of the stomach, taken prior to cytotoxic therapy. The cells are L-dopa decarboxylase (DDC) negative. SNU-1 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 26 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..


CRL-5971 SNU-1 人胃癌細胞


















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 涩涩久久| 亚洲天堂影视 | 午夜精品成年片色多多 | 黄色午夜| 亚洲最新网址 | 欧美啪视频 | 插菊花综合 | 欧美一卡2卡三卡4卡公司免费 | 最新69国产成人精品视频69 | 老子影院午夜久久亚洲 | 色噜噜狠狠先锋影音久久 | 成人国产精品免费视频 | 日韩欧美一区二区三区 | 窝窝人体色 | 黄色短片免费看 | 欧美图片自拍偷拍 | 国产麻豆免费 | 一本大道香蕉大vr在线吗视频 | 日韩欧一级毛片在线播无遮挡 | 图图资源网亚洲综合网站 | 国产福利小视频高清在线观看 | 香蕉视频在线视频 | 玖玖精品在线视频 | 97视频在线播放 | 国产区精品福利在线社区 | 69成人免费视频 | 欧美日韩在线视频观看 | 成人精品在线视频 | 日本视频www色 | 99精品欧美一区二区三区 | 国产成人深夜福利短视频99 | 精品国产一区二区三区久 | 亚洲狠狠婷婷综合久久蜜桃 | 亚洲韩国日本欧美一区二区三区 | 亚洲欧美成人网 | 在线观看中文字幕一区 | 亚洲一区二区三区在线播放 | 亚洲欧美综合日韩字幕v在线 | 午夜秒播 | 99热免费在线观看 | 国产色综合天天综合网 |