日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-5974 SNU-16 人胃癌細胞 腹水

CRL-5974 SNU-16 人胃癌細胞 腹水

簡要描述:CRL-5974 SNU-16 人胃癌細胞 腹水,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,
提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1910

產品分類

Product Category

詳細介紹

CRL-5974 SNU-16 人胃癌細胞 腹水

ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-5974 SNU-16 人胃癌細胞 腹水

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products




ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-5974 SNU-16 人胃癌細胞 腹水

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products



















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 精品一区二区免费视频 | 激情五月开心婷婷 | 动漫免费观看网站 | 天堂在线视频观看 | 成人久久18网站 | 成人 在线欧美亚洲 | yellow中文字幕久久网 | 在线五月婷婷 | 国产精品视频久久久久久 | 国产最新自拍 | 免费观看黄页网站 | 男人阁| va网站| 日日操天天射 | 韩国在线a免费观看网站 | 深夜福利国产精品亚洲尤物 | 狠狠色婷婷综合天天久久丁香 | 亚洲va老文色欧美黄大片人人 | 四虎毛片 | 国产精品99久久久久久董美香 | 亚洲日本中文字幕在线 | 中文字幕一区二区三区精品 | 亚洲综合网在线 | 91免费永久国产在线观看 | 91亚洲视频| 日本一区二区三区在线看 | 日本高清视频成人网www | 亚洲欧美在线看 | 97se狠狠狠狠狼亚洲综合网 | 思99热精品久久只有精品 | 亚洲一区浅井舞香在线播放 | 午夜丁香婷婷 | 欧美成人在线网站 | 毛片一级在线观看 | 91精品婷婷国产综合久久8 | 人体蜈蚣2在线 | 级毛片久久久毛片精品毛片 | 久久久久国产成人精品亚洲午夜 | 大香焦伊人 | 欧洲精品视频在线观看 | 黄色免费在线观看网站 |