日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-5974 SNU-16 人胃癌細胞 腹水

CRL-5974 SNU-16 人胃癌細胞 腹水

簡要描述:CRL-5974 SNU-16 人胃癌細胞 腹水,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,
提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1910

產品分類

Product Category

詳細介紹

CRL-5974 SNU-16 人胃癌細胞 腹水

ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-5974 SNU-16 人胃癌細胞 腹水

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products




ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-5974 SNU-16 人胃癌細胞 腹水

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products



















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 欧美很黄视频在线观看 | 91大神娇喘女神疯狂在线 | 欧美视频第一区 | 免费无遮挡十八女禁污污网站 | 久色伊人 | 成人影片在线免费观看 | 91精品天美精东蜜桃传媒入口 | 国产日本一区二区三区 | 久久99精品久久久久久首页 | 五月婷婷开心综合 | aaa一级黑人毛片 | 成人毛片一区二区三区 | 欧洲视频一区 | 欧美成a人片免费看久久 | 人人干操| 成人三级毛片 | 18一20岁一级毛片 | 免费香蕉成视频成人网 | 两个人的视频在线观看免费完整版 | 亚色在线视频 | 欧美在线播放一区二区 | 中文字幕av一区 | 尤物国午夜精品福利网站 | 国产精品社区在线观看 | 99久久精品国产高清一区二区 | 91在线视频免费 | 四虎欧美在线观看免费 | 受胎岛无删减全集在线观看 | 4虎.最新地址 | 99爱视频99爱在线观看免费 | 欧美不卡视频 | 99久久综合精品国产 | 亚洲高清一区二区三区四区 | 国产原创麻豆 | 国产精品午夜高清在线观看 | 99爱在线视频 | 日本不卡视频免费的 | 日本一区二区三区视频在线 | 国产精品观看视频免费完整版 | 免费韩国美女爽快一级毛片 | 天天做天天爱天天影视综合 |