日本片免费观看一区二区-日本片网址-日本强不卡在线观看-日本青草视频-狠狠色丁香久久综合婷婷-狠狠色丁香六月色

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物腎細(xì)胞>VERO 非洲綠猴腎細(xì)胞

VERO 非洲綠猴腎細(xì)胞

簡要描述:VERO 非洲綠猴腎細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2793

產(chǎn)品分類

Product Category

詳細(xì)介紹

VERO C1008 (E6)非洲綠猴腎細(xì)胞

ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

VERO 非洲綠猴腎細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1586™  Price:
Designations:VERO C1008 [Vero 76, clone E6, Vero E6]

Depositors:EM Earley

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Cercopithecus aethiops

Morphology:epithelial

Source:Organ: kidney
                       Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Susceptibility:Junin virus

Machupo virus

Lassa virus

Marburg virus

Zaire Ebola virus



Comments:This is a clone of VERO 76 (ATCC CRL-1587).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                       Temperature: 37.0°C


Subculturing:Protocol:                        
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
                       Medium Renewal: 2 to 3 times per week


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
                       Storage temperature: liquid nitrogen vapor phase


Doubling Time:22 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020




















 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 91麻精品国产91久久久久 | 久久久久国产精品免费看 | 欧美一区二区三区精品影视 | jizz女人| 日本特黄特色大片免费看 | 岛国大片免费 | 亚洲欧美日韩综合在线播放 | 精品日产一卡2卡三卡4卡在线 | 全天免费91计划网 | 亚洲韩精品欧美一区二区三区 | 免费人成黄页在线观看1024 | 青青在线 | 9久9久女女热精品视频免费观看 | 久久久噜噜噜久久老司机 | 久久99精品久久久久久 | 天天操天天摸天天射 | 精品视频久久久久 | 国产精品无码专区在线观看 | 欧美日韩一区二区三区视频播 | 国产三片高清在线观看 | 免费观看欧美大片 | 国产精品乡下勾搭老头 | 久久这里只有精品6 | 欧美成人午夜视频在线观看 | 污污成人一区二区三区四区 | 99国内精品 | 国产精品v欧美精品∨日韩 国产精品va在线观看一 | 国产高清自拍一区 | 色成人综合网 | 国产成人毛片毛片久久网 | 亚洲最新在线视频 | h视频在线免费观看 | 久久久麻豆 | 日韩精品观看 | 欧洲视频一区 | 免费视频91 | 日本玖玖视频 | 美国大片在线观看 | 91精品成人免费国产 | 欧美影院在线观看在线观看看 | 国产一区二区三区久久 |